Microscopy Facility
Indian Institute of Science Education and Research (IISER) Pune
Leica DM6 B:
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This fully automated upright widefield microscope is equipped with Hamamatsu Orca Flash 4.0 sCMOS Camera.
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Focus: Mechanical and Motorized.
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Light sources: Lumencor Sola Light Engine (LED) for fluorescence imaging with multiple filter sets (DAPI, GFP, RFP and TXRD) and LH LED.
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Capable of BF, DIC, POL and fluorescence imaging.
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Motorized x-y scanning stage.
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A workstation with a copy of the LAS X image analysis software is available for image analysis and processing.
Zeiss Axio Imager. Z1 with ApoTome:
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This upright widefield microscope is equipped with AxioCam HRc (color) and HRm (monochrome CCD) cameras.
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Light sources: X-cite series 120 for fluorescence imaging with multiple filter sets (DAPI, GFP, RFP and DsRed) and HAL 100.
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Capable of Bright field, Darkfield, DIC and fluorescence imaging.
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ApoTome high-resolution capabilities: Structured illumination to achieve thin optical sectioning and diffraction-limited images with high SNR.