Microscopy Facility
Indian Institute of Science Education and Research (IISER) Pune
Stimulated Emission Depletion (STED) Nanoscope:
Leica TCS SP8 STED 3X:
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This inverted laser scanning confocal microscope is equipped with two multi-Alkali PMTs and two Leica HyD hybrid detectors for superior signal-to-noise ratio.
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Light sources: A solid state white light laser for confocal imaging. 592 nm,660 nm, and 775 nm depletion lasers for STED imaging. EL6000 fluorescence illuminator.
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Motorized x-y scanning stage, z-galvo stage and resonant (8000Hz) scanner.
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STED 3D module.
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Combining STED and light-gating principles, this purely optical microscopy technology truly breaks the diffraction limit and can reach an instant resolution down to approx. 50 nm without data processing.
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Live cell imaging with temperature and CO2 control.
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A workstation with a copy of the LAS X image analysis software with Huygens STED deconvolution module is available for image analysis and processing.
Olympus IX81 Live CellTIRF microscope:
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This inverted microscope is equipped with 60x and 100x TIRF lenses, Hamamatsu OrcaR2 CCD and Andor EMCCD cameras.
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Light sources: 405 diode, 491 and 561 lasers and CoolLED pE-4000 illuminator.
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Eight positions high speed excitation filter wheel for fast switching between excitation wavelengths.
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Nose piece Piezo for fast and accurate Z-sectioning.
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Live cell imaging with temperature and CO2 control.
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Operating software: cellSens.