Microscopy Facility
Indian Institute of Science Education and Research (IISER) Pune
Leica TCS DM6 Multiphoton Microscope:
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This upright laser scanning confocal microscope equipped with two multi-Alkali PMTs, one T-PMT, two TLDs, and two HyD-RLDs.
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Light sources: 405 nm, 488 nm and 552 nm lasers for confocal imaging.
Tunable (680-1300 nm) and fixed wavelength (1040 nm)
Femtosecond pulsed lasers for multiphoton and SHG
imaging.
EL6000 fluorescence illuminator.
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Motorized x-y scanning stage, z-galvo stage and resonant (8000Hz) scanner.
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Live cell imaging with temperature and CO2 control.
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Scientifica stage for live animal (intra-vital) imaging.
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A workstation with a copy of the LAS X image analysis software is available for image analysis and processing.
Zeiss LSM 780 Confocal/Multiphoton Microscope:
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This inverted laser scanning confocal microscope is equipped with one GaAsP, two PMTs detectors and one Non-Descanned PMT Detector.
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Light sources: 405 diode, multiline argon (458nm, 488nm, 514nm), 561nm, 594nm and 633nm lasers for confocal imaging Tuneable femtosecond pulsed laser (690-1040 nm) for multiphoton imaging X-Cite 120 series illuminator for fluorescence imaging.
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Motorized x-y scanning stage, z-galvo stage.
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Suitable applications include colocalization studies, immunofluorescence, FRAP, time courses and spectral unmixing.
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Suitable for thick tissue imaging.
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Live cell imaging with temperature and CO2 control.