Microscopy Facility
Indian Institute of Science Education and Research (IISER) Pune
A well-structured monthly training program for the new in-house users to enable them to aptly handle the sophisticated imaging systems.
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Access Control & Training (In-house users ):
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Lecture (2 hours): Basics of microscopy, fluorescence microscopy, and digital imaging.
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System Demonstrations:
1. Basics of Microscopy and Fluorescence microscopy. (2.5 hours ):
This session covers transmitted light microscopy techniques (Bright field, DIC, Phase
contrast, and Darkfield), Kohler Illumination, and Fluorescence microscopy.
Thereafter users get hand-on fluorescence microscopy (Leica DM6) and complete the
assignment.
2. Confocal microscopy (2 hours): Demonstration of confocal microscopy using Zeiss LSM 710.
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Examination: The online MCQ paper needs to be submitted in 45 min. Results are generated instantaneously.
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Confocal Hand-on exercise (2-3 hours): Users complete their assignment (using sub-resolution beads) individually on a LSM 710 confocal microscope on their own.
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Submission of assignments:
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Submission of feedback form (online) and facility access:
We do arrange training sessions for external users upon request. For training-related queries, please get in touch with us- microscopytraining@acads.iiserpune.ac.in