
Microscopy Facility
Indian Institute of Science Education and Research (IISER) Pune

Green: Cis-Golgi - Man II GFP; Red: Trans-Golgi - GalTase RFP. Courtesy of Vibha Singh (N. Balasubramanian Lab) IISER Pune. Citation: Singh et al. (2018). JCS. 2018Â 131:Â jcs215855

Red: BrdU labelled Alexa 568, Blue: DAPI. Courtesy of P. Chandramouli Reddy (Sanjeev Galande Lab), IISER Pune.

Courtesy of Mayurika Lahiri Lab, IISER Pune

Green: Cis-Golgi - Man II GFP; Red: Trans-Golgi - GalTase RFP. Courtesy of Vibha Singh (N. Balasubramanian Lab) IISER Pune. Citation: Singh et al. (2018). JCS. 2018Â 131:Â jcs215855
About the Facility
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This state-of-the-art facility houses a wide spectrum of imaging platforms that allow quantitative measurements and visualization from nanometre sizes to whole tissues and organisms. And from a few milliseconds to hours and days.
Few highlights of the facility include,
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"Super-resolution" imaging techniques (Nobel Prize 2014) that allow molecules to be observed in unprecedented spatial resolution.
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Visualizing living organisms and tissues as they develop over time.
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Visualizing cells at increased depths in tissues and organisms.
The imaging facility has been critical to many of our research outputs, including key insights into cancer development and
progression, how animals and plants develop, the growth and behavior of microbes, and mechanisms underlying
neuropsychiatric disorders. The facility was started in 2010 and has progressed to become one of the pre-eminent
imaging centers in India. Beyond local use, a key activity of the facility is providing specialized training and access to high-
end imaging systems to the nation.